Human Tumor Necrosis Factor Receptor Superfamily Member 10D, TNFRSF10D ELISA Kit

Tumor necrosis factor receptor superfamily member 10D (TNFRSF10D), also known as TNF-related apoptosis-inducing ligand receptor 4 (TRAIL R4), CD264, and Decoy receptor 2, is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis. TRAIL R4/CD264/TNFRSF10D is widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. TRAIL R4/CD264/TNFRSF10D is also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart. The signaling capacity of TRAIL R4 is similar to that of TRAIL R1 and TRAIL R2 with respect to NF-κB activation, but differs in its inability to induce apoptosis. TRAIL R4 retains a C-terminal element containing one third of a consensus death domain motif. Transient overexpression of TRAIL R4 in cells normally sensitive to TRAIL-mediated killing confers complete protection, suggesting that one function of TRAIL R4 may be inhibition of TRAIL cytotoxicity.

Concentration | O.D. | Average | Corrected ------------------------------------------ 15000 pg/ml | 2.357 | 2.27 | 2.182 2.184 7500 pg/ml | 1.203 | 1.148 | 1.06 1.094 3750 pg/ml | 0.618 | 0.599 | 0.511 0.58 1875 pg/ml | 0.348 | 0.343 | 0.255 0.338 937.5 pg/ml | 0.216 | 0.218 | 0.13 0.221 468.75 pg/ml | 0.162 | 0.155 | 0.067 0.148 234.375 pg/ml | 0.129 | 0.124 | 0.036 0.119 0 pg/ml | 0.089 | 0.088 | 0 0.088

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 1015 | 60.4 | 6 Intra-assay | 2 | 20 | 4228 | 242 | 5.7 Intra-assay | 3 | 20 | 10313 | 443 | 4.3 Inter-assay | 1 | 20 | 1008 | 55.6 | 5.5 Inter-assay | 2 | 20 | 4193 | 243 | 5.8 Inter-assay | 3 | 20 | 10340 | 493 | 4.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail