Human Platelet Endothelial Cell Adhesion Molecule, PECAM1 ELISA Kit

The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1200 pg/ml | 2.166 | 2.252 | 2.163 2.338 600 pg/ml | 1.361 | 1.29 | 1.201 1.219 300 pg/ml | 0.761 | 0.722 | 0.633 0.684 150 pg/ml | 0.435 | 0.416 | 0.327 0.398 75 pg/ml | 0.265 | 0.252 | 0.163 0.239 37.5 pg/ml | 0.163 | 0.174 | 0.085 0.186 18.75 pg/ml | 0.123 | 0.127 | 0.038 0.132 0 pg/ml | 0.091 | 0.089 | 0 0.088

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 720 | 35.2 | 4.9 Intra-assay | 2 | 20 | 2322 | 80.1 | 3.4 Intra-assay | 3 | 20 | 6634 | 133 | 2 Inter-assay | 1 | 20 | 711 | 28.5 | 4 Inter-assay | 2 | 20 | 2325 | 64.5 | 2.8 Inter-assay | 3 | 20 | 6685 | 152 | 2.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail