Human Natural Killer Cell Receptor 2B4, CD244 ELISA Kit

The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM) -related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 10000 pg/ml | 2.455 | 2.432 | 2.299 2.409 5000 pg/ml | 1.471 | 1.399 | 1.266 1.327 2500 pg/ml | 0.769 | 0.787 | 0.654 0.804 1250 pg/ml | 0.462 | 0.462 | 0.329 0.461 625 pg/ml | 0.291 | 0.3 | 0.167 0.308 312.5 pg/ml | 0.205 | 0.209 | 0.076 0.212 156.25 pg/ml | 0.177 | 0.172 | 0.039 0.166 0 pg/ml | 0.141 | 0.133 | 0 0.124

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 817 | 22.7 | 2.8 Intra-assay | 2 | 20 | 2917 | 83.7 | 2.9 Intra-assay | 3 | 20 | 5833 | 184 | 3.2 Inter-assay | 1 | 20 | 820 | 57.4 | 7 Inter-assay | 2 | 20 | 2910 | 191 | 6.6 Inter-assay | 3 | 20 | 5853 | 373 | 6.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail