Human Slam Family Member 5, CD84 ELISA Kit

The CD2 family receptors are type I transmembrane glycoproteins belonging to immunoglobulin (Ig) superfamily characterized by a membrane-proximal Ig constant 2 (C2) domain and a membrane-distal variable (V) domain that is responsible for ligand recognition. CD84, also known as LY9B and SLAMF5, is a homophilic member of the SLAM (signaling lymphocyte activation molecule) subfamily of the CD2 family. The SLAM family receptorsmediate signal transduction through the interaction of its ITSM (immunoreceptor tyrosine-based switch motifs) in the intracellular region and the SH2 domain of adaptor molecules SAP (SLAM-associated protein) and EAT-2 (EWS-activated transcript 2), and accordingly modulate both adaptive and innate immune responses. The CD84-CD84 interaction was independent of its cytoplasmic tail. Thus, CD84 is its own ligand and acts as a costimulatory molecule. CD84 is expressed on cells from almost all hematopoietic lineages and on CD34+ hematopoietic progenitor cells, suggesting that CD84 serves as a marker for committed hematopoietic progenitor cells.

Concentration | O.D. | Average | Corrected ------------------------------------------ 10000 pg/ml | 1.673 | 1.659 | 1.551 1.646 5000 pg/ml | 1.024 | 1.012 | 0.904 1 2500 pg/ml | 0.648 | 0.617 | 0.509 0.587 1250 pg/ml | 0.365 | 0.368 | 0.26 0.372 625 pg/ml | 0.234 | 0.238 | 0.13 0.243 312.5 pg/ml | 0.167 | 0.171 | 0.063 0.176 156.25 pg/ml | 0.14 | 0.142 | 0.034 0.144 0 pg/ml | 0.103 | 0.108 | 0 0.114

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 632 | 38 | 6 Intra-assay | 2 | 20 | 1620 | 57.7 | 3.6 Intra-assay | 3 | 20 | 5787 | 253 | 4.4 Inter-assay | 1 | 20 | 629 | 59.5 | 9.5 Inter-assay | 2 | 20 | 1625 | 79.3 | 4.9 Inter-assay | 3 | 20 | 5772 | 265 | 4.6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail