Human Polymeric Immunoglobulin Receptor, PIGR ELISA Kit

Polymeric immunoglobulin receptor, also known as PIGR, is a member of the immunoglobulin superfamily and a Fc receptor. The ectodomain of this receptor consists of five units with homology to the variable units of immunoglobulins and a transmembrane region, which also has some homology to certain immunoglobulin variable regions. PIGR is expressed on several glandular epithelia including those of liver and breast. The deduced amino-acid sequence has a length of 764 residues and shows an overall similarity of 56% and 64% with the rabbit and rat counterpart. PIGR mediates transcellular transport of polymeric immunoglobulin molecules, and thus facilitates the secretion of IgA and IgM. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment of PIGR.

Concentration | O.D. | Average | Corrected ------------------------------------------ 10000 pg/ml | 2.535 | 2.485 | 2.364 2.435 5000 pg/ml | 1.324 | 1.289 | 1.168 1.254 2500 pg/ml | 0.604 | 0.644 | 0.523 0.683 1250 pg/ml | 0.406 | 0.391 | 0.27 0.376 625 pg/ml | 0.262 | 0.249 | 0.128 0.236 312.5 pg/ml | 0.172 | 0.183 | 0.062 0.194 156.25 pg/ml | 0.154 | 0.157 | 0.036 0.16 0 pg/ml | 0.116 | 0.121 | 0 0.126

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 499 | 46.2 | 9.3 Intra-assay | 2 | 20 | 1994 | 84.2 | 4.2 Intra-assay | 3 | 20 | 7387 | 321 | 4.3 Inter-assay | 1 | 20 | 495 | 30.1 | 6.1 Inter-assay | 2 | 20 | 1991 | 88 | 4.4 Inter-assay | 3 | 20 | 7469 | 362 | 4.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail