Human High Affinity Nerve Growth Factor Receptor, NTRK1 ELISA Kit

TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by the pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in the TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation, and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF) . Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as the establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 10000 pg/ml | 2.262 | 2.246 | 2.16 2.23 5000 pg/ml | 1.166 | 1.152 | 1.066 1.139 2500 pg/ml | 0.564 | 0.6 | 0.514 0.636 1250 pg/ml | 0.339 | 0.332 | 0.246 0.326 625 pg/ml | 0.198 | 0.202 | 0.116 0.206 312.5 pg/ml | 0.136 | 0.143 | 0.057 0.15 156.25 pg/ml | 0.104 | 0.108 | 0.022 0.113 0 pg/ml | 0.082 | 0.086 | 0 0.091

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 1039 | 90.3 | 8.7 Intra-assay | 2 | 20 | 3246 | 240 | 7.4 Intra-assay | 3 | 20 | 6492 | 526 | 8.1 Inter-assay | 1 | 20 | 1043 | 75.9 | 7.3 Inter-assay | 2 | 20 | 3209 | 257 | 8 Inter-assay | 3 | 20 | 6526 | 449 | 6.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail