Human Npc Intracellular Cholesterol Transporter 2, NPC2 ELISA Kit

Niemann-Pick Type C2 (NPC2) plays an important role in the regulation of intracellular cholesterol homeostasis via direct binding with free cholesterol. NPC2 is an intralysosomal protein that binds cholesterol in vitro. NPC2 is a small lysosomal glycoprotein that binds cholesterol with submicromolar affinity. Deficiency in NPC2 is the cause of Niemann-Pick type C2 disease, a fatal neurovisceral disorder characterized by the accumulation of cholesterol in lysosomes. Niemann-Pick disease, type C2 (NPC2) protein is one of the most abundant components of the epididymal fluid and contains a functional cholesterol-binding site that can transfer cholesterol between membranes, it has been suggested for years that NPC2 could be involved in the regulation of cholesterol levels in spermatozoa during epididymal maturation.

Concentration | O.D. | Average | Corrected ------------------------------------------ 10000 pg/ml | 2.718 | 2.59 | 2.464 2.463 5000 pg/ml | 1.426 | 1.449 | 1.323 1.473 2500 pg/ml | 0.838 | 0.794 | 0.668 0.751 1250 pg/ml | 0.445 | 0.468 | 0.342 0.491 625 pg/ml | 0.28 | 0.298 | 0.172 0.317 312.5 pg/ml | 0.203 | 0.214 | 0.088 0.225 156.25 pg/ml | 0.163 | 0.17 | 0.044 0.178 0 pg/ml | 0.132 | 0.126 | 0 0.12

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 340 | 23.5 | 6.9 Intra-assay | 2 | 20 | 1701 | 90 | 5.3 Intra-assay | 3 | 20 | 6074 | 179 | 2.9 Inter-assay | 1 | 20 | 338 | 27.2 | 8.1 Inter-assay | 2 | 20 | 1712 | 119 | 7 Inter-assay | 3 | 20 | 6030 | 359 | 6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail