Mouse Hematopoietic Progenitor Cell Antigen Cd34, Cd34 ELISA Kit

Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.

Concentration | O.D. | Average | Corrected ------------------------------------------ 10000 pg/ml | 2.357 | 2.443 | 2.348 2.529 5000 pg/ml | 1.185 | 1.176 | 1.081 1.167 2500 pg/ml | 0.58 | 0.603 | 0.508 0.626 1250 pg/ml | 0.311 | 0.333 | 0.238 0.355 625 pg/ml | 0.197 | 0.211 | 0.116 0.224 312.5 pg/ml | 0.146 | 0.15 | 0.055 0.154 156.25 pg/ml | 0.114 | 0.117 | 0.022 0.12 0 pg/ml | 0.094 | 0.095 | 0 0.096

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 1123 | 44.1 | 3.9 Intra-assay | 2 | 20 | 2495 | 89.6 | 3.6 Intra-assay | 3 | 20 | 5940 | 281 | 4.7 Inter-assay | 1 | 20 | 1132 | 93.7 | 8.3 Inter-assay | 2 | 20 | 2523 | 161 | 6.4 Inter-assay | 3 | 20 | 5896 | 350 | 5.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail