Mouse Prostatic Acid Phosphatase, Acp3 ELISA Kit

Prostatic acid phosphatase (PAP, or ACPP), also known as prostatic specific acid phosphatase (PSAP), is an enzyme produced by the prostate. As a non-specific phosphomonoesterase, Prostatic acid phosphatase is synthesized and secreted into seminal plasma under androgenic control. The enzyme is a dimer of molecular weight around 100 kDa. Prostatic acid phosphatase is a clinically important protein for its relevance as a biomarker of prostate carcinoma. Furthermore, it has a potential role in fertilization. The major action of PAP is to dephosphorylate macromolecules with the help of catalytic residues (His (12) and Asp (258) ) that are located in the cleft between two domains. Cellular prostatic acid phosphatase (cPAcP), an authentic tyrosine phosphatase, is proposed to function as a negative growth regulator of prostate cancer (PCa) cells in part through its dephosphorylation of ErbB-2. cPAcP functions as a neutral protein tyrosine phosphatase (PTP) in prostate cancer cells and dephosphorylates HER-2/ErbB-2/Neu (HER-2: human epidermal growth factor receptor-2) at the phosphotyrosine (p-Tyr) residues. Injection of the secretory isoform of PAP has potent antinociceptive effects in mouse models of chronic pain. This enzyme exhibits ecto-5'-nucleotidase activity, is widely distributed, and implicated in the formation of chronic pain. Additionally, PAP could be a target molecule in specific immunotherapy for patients with nonprostate adenocarcinomas including colon and gastric cancers.

Concentration | O.D. | Average | Corrected ------------------------------------------ 10000 pg/ml | 2.286 | 2.352 | 2.244 2.418 5000 pg/ml | 1.317 | 1.271 | 1.163 1.225 2500 pg/ml | 0.721 | 0.699 | 0.591 0.678 1250 pg/ml | 0.395 | 0.412 | 0.304 0.43 625 pg/ml | 0.257 | 0.258 | 0.15 0.259 312.5 pg/ml | 0.176 | 0.182 | 0.074 0.189 156.25 pg/ml | 0.153 | 0.144 | 0.036 0.136 0 pg/ml | 0.107 | 0.108 | 0 0.11

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 538 | 17.5 | 3.3 Intra-assay | 2 | 20 | 1920 | 62.9 | 3.3 Intra-assay | 3 | 20 | 7681 | 259 | 3.4 Inter-assay | 1 | 20 | 544 | 55.5 | 10.2 Inter-assay | 2 | 20 | 1942 | 140 | 7.2 Inter-assay | 3 | 20 | 7638 | 535 | 7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail