Human Interleukin-22 Receptor Subunit Alpha-2, IL22RA2 ELISA Kit

Interleukin-22 receptor subunit alpha-2 (IL-22RA2), also known as interleukin-22-binding protein (IL-22BP), is a subunit of the receptor for interleukin 22. IL-22BP belongs to the type II cytokine receptor family and contains 3 fibronectin type-III domains. IL-22BP/IL-22RA2 is expressed in a range of tissues, including those in the digestive, female reproductive, and immune systems. It is expressed in the placenta, spleen, breast, skin, and lung. It is also detected in the intestinal tract, testis, brain, heart, and thymus. The dominant cell types expressing IL-22BP/IL-22RA2 were mononuclear cells and epithelium. IL-22BP/IL-22RA2 may play an important role as an IL-22 antagonist in the regulation of inflammatory responses. Interleukin-22 (IL-22) is a member of the IL-10 family. It is produced by T cells and induces the production of acute-phase reactants. IL-22 plays important role in the immune response through activation of the STAT 3 signal transduction pathway. Two types of IL-22-binding receptors have been discovered, a membrane-bound receptor and a soluble receptor.

Concentration | O.D. | Average | Corrected ------------------------------------------ 8500 pg/ml | 2.183 | 2.23 | 2.141 2.278 4250 pg/ml | 1.355 | 1.281 | 1.192 1.207 2125 pg/ml | 0.691 | 0.672 | 0.583 0.654 1062.5 pg/ml | 0.376 | 0.388 | 0.299 0.4 531.25 pg/ml | 0.251 | 0.247 | 0.158 0.244 265.625 pg/ml | 0.173 | 0.163 | 0.074 0.154 132.8125 pg/ml | 0.124 | 0.121 | 0.032 0.119 0 pg/ml | 0.086 | 0.089 | 0 0.093

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 921 | 33.2 | 3.6 Intra-assay | 2 | 20 | 2046 | 66.8 | 3.3 Intra-assay | 3 | 20 | 5529 | 195 | 3.5 Inter-assay | 1 | 20 | 912 | 52.6 | 5.8 Inter-assay | 2 | 20 | 2039 | 94.9 | 4.7 Inter-assay | 3 | 20 | 5560 | 214 | 3.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail