Human Carbonic Anhydrase 14, CA14 ELISA Kit

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29–46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.

Concentration | O.D. | Average | Corrected ------------------------------------------ 8000 pg/ml | 2.602 | 2.635 | 2.523 2.668 4000 pg/ml | 1.441 | 1.413 | 1.301 1.385 2000 pg/ml | 0.745 | 0.773 | 0.661 0.801 1000 pg/ml | 0.448 | 0.434 | 0.322 0.42 500 pg/ml | 0.28 | 0.278 | 0.166 0.276 250 pg/ml | 0.201 | 0.196 | 0.084 0.191 125 pg/ml | 0.147 | 0.157 | 0.045 0.166 0 pg/ml | 0.108 | 0.112 | 0 0.116

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 403 | 18.1 | 4.5 Intra-assay | 2 | 20 | 1754 | 82.2 | 4.7 Intra-assay | 3 | 20 | 4873 | 228 | 4.7 Inter-assay | 1 | 20 | 407 | 33.3 | 8.2 Inter-assay | 2 | 20 | 1773 | 95.1 | 5.4 Inter-assay | 3 | 20 | 4910 | 254 | 5.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail