Human Apolipoprotein A-I, APOA1 ELISA Kit

Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease.

Concentration | O.D. | Average | Corrected ------------------------------------------ 8000 pg/ml | 2.284 | 2.26 | 2.113 2.236 4000 pg/ml | 1.189 | 1.183 | 1.036 1.177 2000 pg/ml | 0.576 | 0.564 | 0.417 0.552 1000 pg/ml | 0.329 | 0.337 | 0.19 0.345 500 pg/ml | 0.225 | 0.231 | 0.084 0.237 250 pg/ml | 0.17 | 0.175 | 0.028 0.18 0 pg/ml | 0.146 | 0.147 | 0 0.148

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 449 | 14.7 | 3.3 Intra-assay | 2 | 20 | 1547 | 53 | 3.4 Intra-assay | 3 | 20 | 4990 | 176 | 3.5 Inter-assay | 1 | 20 | 453 | 46.3 | 10.2 Inter-assay | 2 | 20 | 1544 | 113 | 7.3 Inter-assay | 3 | 20 | 4964 | 350 | 7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail