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Canine Hepatitis A Virus Cellular Receptor 1 ELISA Kit

Product Specifications

Background

HAV cellular receptor 1 (HAVCR1), also known as Kidney injury molecule 1 (KIM-1) and T cell immunoglobulin mucin 1 (TIM-1), is a type of integral membrane glycoprotein. KIM-1 protein is widely expressed with the highest levels in the kidney and testis. It has been shown to play a major role as a human susceptibility gene for asthma, allergy, and autoimmunity. IgA1lambda is a specific ligand of KIM-1 protein and that their association has a synergistic effect in virus-receptor interactions. KIM-1 involves in the pathogenesis of acute kidney injury. It had been confirmed that KIM-1 is a human urinary renal dysfunction biomarker. Moreover, KIM-1 protein is a novel regulatory molecule of flow-induced calcium signaling.

Product Name Alternative

Hepatitis A virus cellular receptor 1

Synonyms

CD365; HAVCR; HAVCR 1; KIM 1; TIM; TIM1; TIMD 1

Target

TIM-1

Field of Research

Immunology, Microbiology

Assay Type

Sandwich

Assay Protocol

Concentration | O.D. | Average | Corrected ------------------------------------------ 8000 pg/ml | 1.9 | 1.884 | 1.757 1.868 4000 pg/ml | 1.197 | 1.216 | 1.089 1.236 2000 pg/ml | 0.728 | 0.746 | 0.619 0.764 1000 pg/ml | 0.465 | 0.463 | 0.336 0.462 500 pg/ml | 0.288 | 0.303 | 0.176 0.319 250 pg/ml | 0.197 | 0.21 | 0.083 0.224 125 pg/ml | 0.172 | 0.166 | 0.039 0.16 0 pg/ml | 0.127 | 0.127 | 0 0.128

Assay Performance Time

3.5h

Sample Type

Serum, plasma, cell culture supernates

Detection Range

125-8000 pg/mL

Precision

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 1152 | 65.4 | 5.7 Intra-assay | 2 | 20 | 2743 | 131 | 4.8 Intra-assay | 3 | 20 | 5714 | 289 | 5.1 Inter-assay | 1 | 20 | 1150 | 60.7 | 5.3 Inter-assay | 2 | 20 | 2752 | 117 | 4.2 Inter-assay | 3 | 20 | 5706 | 409 | 7.2

Sensitivity

34.06 pg/mL

Components

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail

Storage Temperature

2-8°C

Species

Canine

Available Sizes

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