Human Cadherin-16, CDH16 ELISA Kit

KSP-Cadherin/Cadherin-16 is a member of the cadherin superfamily, calcium-dependent, membrane-associated glycoproteins. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. KSP-Cadherin/Cadherin-16 can be detected at later stages of tubulogenesis during human renal development and in the distal tubules of adult kidneys, no expression was found by immunohistochemistry or Western blot analysis in RCC tumour tissues and several RCC cell lines. However, despite the lack of protein expression, mRNA synthesis of KSP-Cadherin/Cadherin-16 could be detected by reverse transcriptase-polymerase chain reaction analysis in all RCC tissues and most of the RCC cell lines studied, although at a reduced level. The loss of KSP-Cadherin/Cadherin-16 protein was only observed in the malignant part of the tumour kidneys, whereas in the normal part of the affected kidneys KSP-Cadherin/Cadherin-16 expression was clearly detected. These results indicate a downregulation of Ksp-cadherin in RCC and suggest a role for this cell adhesion molecule in tumour suppression.

Concentration | O.D. | Average | Corrected ------------------------------------------ 7000 pg/ml | 2.242 | 2.3 | 2.218 2.359 3500 pg/ml | 1.295 | 1.362 | 1.28 1.43 1750 pg/ml | 0.713 | 0.706 | 0.624 0.699 875 pg/ml | 0.409 | 0.429 | 0.347 0.45 437.5 pg/ml | 0.255 | 0.247 | 0.165 0.24 218.75 pg/ml | 0.167 | 0.163 | 0.081 0.16 109.375 pg/ml | 0.123 | 0.117 | 0.035 0.111 0 pg/ml | 0.077 | 0.082 | 0 0.088

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 309 | 18.7 | 6 Intra-assay | 2 | 20 | 965 | 55.1 | 5.7 Intra-assay | 3 | 20 | 4384 | 304 | 6.9 Inter-assay | 1 | 20 | 307 | 23.9 | 7.8 Inter-assay | 2 | 20 | 977 | 98.5 | 10.1 Inter-assay | 3 | 20 | 4399 | 282 | 6.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail