Human Cadherin-5, CDH5 ELISA Kit

Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, an endothelial specific cell-cell adhesion molecule, plays a pivotal role in the formation, maturation and remodeling of the vascular wall. VE-Cadherin is widely considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. This specificity of VE-cadherin for vascular endothelial cells is important not only in blood and lymph vessel biology and medicine, but also for cell-type-based diagnoses, notably those of metastatic tumors. As a classical cadherin, VE-Cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Mechanisms that regulate VE-cadherin-mediated adhesion are important for the control of vascular permeability and leukocyte extravasation. In addition to its adhesive functions, VE-Cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Consequently, VE-cadherin is essential during embryonic angiogenesis.

Concentration | O.D. | Average | Corrected ------------------------------------------ 6000 pg/ml | 2.4 | 2.349 | 2.232 2.297 3000 pg/ml | 1.317 | 1.287 | 1.17 1.256 1500 pg/ml | 0.659 | 0.7 | 0.583 0.74 750 pg/ml | 0.409 | 0.404 | 0.287 0.398 375 pg/ml | 0.273 | 0.262 | 0.145 0.251 187.5 pg/ml | 0.193 | 0.187 | 0.07 0.18 93.75 pg/ml | 0.16 | 0.15 | 0.033 0.139 0 pg/ml | 0.112 | 0.117 | 0 0.122

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 464 | 34.6 | 7.5 Intra-assay | 2 | 20 | 947 | 53.6 | 5.7 Intra-assay | 3 | 20 | 3157 | 123 | 3.9 Inter-assay | 1 | 20 | 461 | 24.3 | 5.3 Inter-assay | 2 | 20 | 952 | 51.1 | 5.4 Inter-assay | 3 | 20 | 3147 | 159 | 5.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail