Human STK4 ELISA Kit

Dysregulation of MST1/STK4, a key kinase component of the Hippo-YAP pathway, is linked to the etiology of many cancers with poor prognosis. STK4/Hippo pathway may have important therapeutic implications for cancer. The tumor suppressor serine/threonine-protein kinase 4 (STK4) differentially regulates TLR3/4/9-mediated inflammatory responses in macrophages and thereby is protective against chronic inflammation-associated Hepatocellular carcinoma (HCC) . STK4 has potential as a diagnostic biomarker and therapeutic target for inflammation-induced HCC.

Concentration | O.D. | Average | Corrected ------------------------------------------ 6000 pg/ml | 2.279 | 2.323 | 2.206 2.366 3000 pg/ml | 1.273 | 1.314 | 1.197 1.354 1500 pg/ml | 0.77 | 0.76 | 0.643 0.749 750 pg/ml | 0.417 | 0.436 | 0.319 0.454 375 pg/ml | 0.278 | 0.269 | 0.152 0.26 187.5 pg/ml | 0.183 | 0.185 | 0.068 0.186 93.75 pg/ml | 0.164 | 0.156 | 0.039 0.147 0 pg/ml | 0.109 | 0.117 | 0 0.125

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 323 | 25.5 | 7.9 Intra-assay | 2 | 20 | 1010 | 49.7 | 4.9 Intra-assay | 3 | 20 | 4589 | 154 | 3.4 Inter-assay | 1 | 20 | 325 | 28.4 | 8.8 Inter-assay | 2 | 20 | 1003 | 69.1 | 6.9 Inter-assay | 3 | 20 | 4597 | 274 | 6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail