Mouse Tumor Necrosis Factor, Tnf ELISA Kit

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1) . TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & Antibody Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 6000 pg/ml | 2.407 | 2.523 | 2.392 2.639 3000 pg/ml | 1.466 | 1.474 | 1.343 1.482 1500 pg/ml | 0.865 | 0.919 | 0.788 0.973 750 pg/ml | 0.546 | 0.553 | 0.422 0.56 375 pg/ml | 0.341 | 0.357 | 0.226 0.373 187.5 pg/ml | 0.249 | 0.248 | 0.117 0.248 93.75 pg/ml | 0.189 | 0.186 | 0.055 0.183 0 pg/ml | 0.138 | 0.131 | 0 0.124

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 252 | 16.6 | 6.6 Intra-assay | 2 | 20 | 811 | 37.8 | 4.7 Intra-assay | 3 | 20 | 3528 | 107 | 3 Inter-assay | 1 | 20 | 254 | 18.4 | 7.3 Inter-assay | 2 | 20 | 814 | 42.2 | 5.2 Inter-assay | 3 | 20 | 3544 | 159 | 4.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail