Human Apoptosis Regulator Bcl-2, BCL2 ELISA Kit

BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 5000 pg/ml | 2.557 | 2.488 | 2.388 2.419 2500 pg/ml | 1.379 | 1.441 | 1.341 1.503 1250 pg/ml | 0.737 | 0.728 | 0.628 0.719 625 pg/ml | 0.408 | 0.393 | 0.293 0.378 312.5 pg/ml | 0.252 | 0.24 | 0.14 0.228 156.25 pg/ml | 0.164 | 0.167 | 0.067 0.17 78.125 pg/ml | 0.129 | 0.136 | 0.036 0.143 0 pg/ml | 0.096 | 0.1 | 0 0.104

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 288 | 26.4 | 9.2 Intra-assay | 2 | 20 | 779 | 32.7 | 4.2 Intra-assay | 3 | 20 | 3388 | 141 | 4.2 Inter-assay | 1 | 20 | 287 | 17.7 | 6.2 Inter-assay | 2 | 20 | 785 | 36 | 4.6 Inter-assay | 3 | 20 | 3355 | 160 | 4.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail