Human Tyrosine-Protein Kinase Receptor Tie-1, TIE1 ELISA Kit

Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 also known as Tie1 is an angiopoietin receptor and is an orphan receptor tyrosine kinase that is expressed almost exclusively in endothelial cells and that is required for normal embryonic vascular development. The receptor tyrosine kinase Tie1 is expressed primarily in vascular endothelial cells. The receptor has also been detected in epithelial tumours in breast, thyroid and gastric cancers and in tumour cell lines where it appears as a 45 kDa truncated receptor fragment. Tie1 promotes endothelial cell survival, but other studies have suggested that the Tie1 kinase has little to no activity. Embryos deficient in Tie1 failed to establish structural integrity of vascular endothelial cells, resulting in oedema and subsequently localized haemorrhage. Tie1 is significantly higher in human aortic endothelial cells than in human umbilical vein endothelial cells. Additionally, attachment of cells of monocytic lineage to endothelial cells is also enhanced by Tie1 expression. Collectively Tie1 has a proinflammatory property and may play a role in endothelial inflammatory diseases such as atherosclerosis.

Concentration | O.D. | Average | Corrected ------------------------------------------ 5000 pg/ml | 3.523 | 3.44 | 3.308 3.358 2500 pg/ml | 1.721 | 1.811 | 1.679 1.902 1250 pg/ml | 0.964 | 0.906 | 0.774 0.849 625 pg/ml | 0.494 | 0.497 | 0.365 0.501 312.5 pg/ml | 0.3 | 0.313 | 0.181 0.326 156.25 pg/ml | 0.232 | 0.226 | 0.094 0.221 78.125 pg/ml | 0.185 | 0.176 | 0.044 0.168 0 pg/ml | 0.138 | 0.132 | 0 0.127

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 294 | 22.7 | 7.7 Intra-assay | 2 | 20 | 717 | 38.9 | 5.4 Intra-assay | 3 | 20 | 2867 | 120 | 4.2 Inter-assay | 1 | 20 | 295 | 31.3 | 10.6 Inter-assay | 2 | 20 | 721 | 61 | 8.5 Inter-assay | 3 | 20 | 2901 | 204 | 7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail