Human Angiopoietin-1 Receptor, TEK ELISA Kit

TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Concentration | O.D. | Average | Corrected ------------------------------------------ 5000 pg/ml | 2.68 | 2.599 | 2.508 2.518 2500 pg/ml | 1.525 | 1.5 | 1.409 1.475 1250 pg/ml | 0.83 | 0.832 | 0.741 0.834 625 pg/ml | 0.432 | 0.447 | 0.356 0.461 312.5 pg/ml | 0.28 | 0.267 | 0.176 0.254 156.25 pg/ml | 0.181 | 0.177 | 0.086 0.173 78.125 pg/ml | 0.143 | 0.134 | 0.043 0.125 0 pg/ml | 0.094 | 0.091 | 0 0.088

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 266 | 14.5 | 5.5 Intra-assay | 2 | 20 | 1024 | 47.8 | 4.7 Intra-assay | 3 | 20 | 2925 | 63.4 | 2.2 Inter-assay | 1 | 20 | 265 | 20.5 | 7.7 Inter-assay | 2 | 20 | 1015 | 79 | 7.8 Inter-assay | 3 | 20 | 2941 | 188 | 6.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail