Human T-Cell Surface Glycoprotein Cd8 Alpha Chain, CD8A ELISA Kit

T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

Concentration | O.D. | Average | Corrected ------------------------------------------ 5000 pg/ml | 1.829 | 1.835 | 1.742 1.842 2500 pg/ml | 1.125 | 1.107 | 1.014 1.09 1250 pg/ml | 0.664 | 0.642 | 0.549 0.62 625 pg/ml | 0.353 | 0.369 | 0.276 0.386 312.5 pg/ml | 0.231 | 0.233 | 0.14 0.236 156.25 pg/ml | 0.169 | 0.163 | 0.07 0.157 78.125 pg/ml | 0.129 | 0.132 | 0.039 0.136 0 pg/ml | 0.09 | 0.093 | 0 0.097

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 306 | 17.7 | 5.8 Intra-assay | 2 | 20 | 900 | 31.8 | 3.5 Intra-assay | 3 | 20 | 3104 | 165 | 5.3 Inter-assay | 1 | 20 | 307 | 19.6 | 6.4 Inter-assay | 2 | 20 | 898 | 92.2 | 10.3 Inter-assay | 3 | 20 | 3132 | 104 | 3.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail