Human Lysyl Oxidase Homolog 2, LOXL2 ELISA Kit

Lysyl oxidase homolog 2, also known as Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14 and LOXL2, is a secreted protein that belongs to the lysyl oxidase family. LOXL2 contains four SRCR domains. The lysyl oxidase family is made up of five members: lysyl oxidase (LOX) and lysyl oxidase-like 1-4 (LOXL1, LOXL2, LOXL3, LOXL4) . All members share conserved C-terminal catalytic domains that provide for lysyl oxidase or lysyl oxidase-like enzyme activity; and more divergent propeptide regions. LOX family enzyme activities catalyze the final enzymatic conversion required for the formation of normal biosynthetic collagen and elastin cross-links. LOXL2 is expressed by pre-hypertrophic and hypertrophic chondrocytes in vivo, and that LOXL2 expression is regulated in vitro as a function of chondrocyte differentiation. LOXL2 promotes chondrocyte differentiation by mechanisms that are likely to include roles as both a regulator and an effector of chondrocyte differentiation. LOXL2 expression could also be explored as a molecular target in the prevention of breast cancer progression.

Concentration | O.D. | Average | Corrected ------------------------------------------ 5000 pg/ml | 2.501 | 2.492 | 2.402 2.483 2500 pg/ml | 1.338 | 1.399 | 1.309 1.46 1250 pg/ml | 0.782 | 0.808 | 0.718 0.834 625 pg/ml | 0.436 | 0.449 | 0.359 0.462 312.5 pg/ml | 0.271 | 0.265 | 0.175 0.259 156.25 pg/ml | 0.155 | 0.163 | 0.073 0.171 78.125 pg/ml | 0.127 | 0.123 | 0.033 0.119 0 pg/ml | 0.092 | 0.09 | 0 0.088

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 369 | 17.5 | 4.7 Intra-assay | 2 | 20 | 1604 | 79.9 | 5 Intra-assay | 3 | 20 | 3818 | 173 | 4.5 Inter-assay | 1 | 20 | 366 | 29.7 | 8.1 Inter-assay | 2 | 20 | 1585 | 139 | 8.8 Inter-assay | 3 | 20 | 3793 | 284 | 7.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail