Human Cd82 Antigen, CD82 ELISA Kit

CD82, also known as KAI-1, structurally belongs to tetraspanin family while categorised as metastasis suppressor gene on functional grounds. KAI1/CD82 is localized on cell membrane and form interactions with other tetraspanins, integrins and chemokines which are respectively responsible for cell migration, adhesion and signalling. Downregulation of CD82 expression is associated with the advanced stages of many human cancers and correlates with the acquisition of metastatic potential. Recent studies suggest that complex mechanisms underlie CD82 loss of function, including altered transcriptional regulation, splice variant production and post-translational protein modifications, and indicate a central role for CD82 in controlling metastasis as a 'molecular facilitator'. The loss of KAI1/CD82 expression in invasive and metastatic cancers is due to a complex, epigenetic mechanism that probably involves transcription factors such as NFkappaB, p53, and beta-catenin. A loss of KAI1 expression is also associated with the advanced stages of many human malignancies and results in the acquisition of invasive and metastatic capabilities by tumour cells. Thus, KAI1/CD82 is regarded as a wide-spectrum tumor metastasis suppressor.

Concentration | O.D. | Average | Corrected ------------------------------------------ 5000 pg/ml | 2.655 | 2.531 | 2.412 2.408 2500 pg/ml | 1.43 | 1.362 | 1.243 1.294 1250 pg/ml | 0.702 | 0.671 | 0.552 0.641 625 pg/ml | 0.407 | 0.382 | 0.263 0.357 312.5 pg/ml | 0.279 | 0.281 | 0.162 0.283 156.25 pg/ml | 0.227 | 0.217 | 0.098 0.208 78.125 pg/ml | 0.167 | 0.161 | 0.042 0.156 0 pg/ml | 0.124 | 0.119 | 0 0.115

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 512 | 27.5 | 5.4 Intra-assay | 2 | 20 | 1424 | 44.3 | 3.1 Intra-assay | 3 | 20 | 3559 | 198 | 5.6 Inter-assay | 1 | 20 | 509 | 17 | 3.3 Inter-assay | 2 | 20 | 1422 | 118 | 8.3 Inter-assay | 3 | 20 | 3566 | 123 | 3.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail