Ferret Tumor Necrosis Factor Alpha ELISA Kit

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1) . TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment

Concentration | O.D. | Average | Corrected ------------------------------------------ 5000 pg/ml | 2.462 | 2.367 | 2.252 2.272 2500 pg/ml | 1.187 | 1.154 | 1.039 1.121 1250 pg/ml | 0.549 | 0.556 | 0.441 0.562 625 pg/ml | 0.294 | 0.305 | 0.19 0.315 312.5 pg/ml | 0.197 | 0.187 | 0.072 0.177 156.25 pg/ml | 0.157 | 0.147 | 0.032 0.137 78.125 pg/ml | 0.134 | 0.129 | 0.014 0.124 0 pg/ml | 0.107 | 0.115 | 0 0.123

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 469 | 18.2 | 3.9 Intra-assay | 2 | 20 | 1234 | 41.5 | 3.4 Intra-assay | 3 | 20 | 3010 | 143 | 4.8 Inter-assay | 1 | 20 | 474 | 38.8 | 8.2 Inter-assay | 2 | 20 | 1223 | 80.6 | 6.6 Inter-assay | 3 | 20 | 2991 | 176 | 5.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail