Human Mhc Class I Polypeptide-Related Sequence B, MICB ELISA Kit

MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without the capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B is minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.

Concentration | O.D. | Average | Corrected ------------------------------------------ 5000 pg/ml | 2.213 | 2.321 | 2.233 2.429 2500 pg/ml | 1.269 | 1.274 | 1.186 1.279 1250 pg/ml | 0.717 | 0.695 | 0.607 0.674 625 pg/ml | 0.394 | 0.376 | 0.288 0.358 312.5 pg/ml | 0.263 | 0.246 | 0.158 0.229 156.25 pg/ml | 0.17 | 0.169 | 0.081 0.168 78.125 pg/ml | 0.136 | 0.128 | 0.04 0.12 0 pg/ml | 0.092 | 0.088 | 0 0.084

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 385 | 21.1 | 5.5 Intra-assay | 2 | 20 | 802 | 26.2 | 3.3 Intra-assay | 3 | 20 | 2767 | 62.8 | 2.3 Inter-assay | 1 | 20 | 389 | 21.5 | 5.5 Inter-assay | 2 | 20 | 792 | 42.6 | 5.4 Inter-assay | 3 | 20 | 2790 | 137 | 4.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail