Human Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1, CEACAM1 ELISA Kit

The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: ICC Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 5000 pg/ml | 2.648 | 2.571 | 2.447 2.495 2500 pg/ml | 1.48 | 1.486 | 1.362 1.492 1250 pg/ml | 0.795 | 0.83 | 0.706 0.865 625 pg/ml | 0.468 | 0.473 | 0.349 0.478 312.5 pg/ml | 0.294 | 0.304 | 0.18 0.314 156.25 pg/ml | 0.223 | 0.219 | 0.095 0.215 78.125 pg/ml | 0.176 | 0.166 | 0.042 0.157 0 pg/ml | 0.125 | 0.124 | 0 0.124

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 255 | 17.6 | 6.9 Intra-assay | 2 | 20 | 531 | 18.1 | 3.4 Intra-assay | 3 | 20 | 2654 | 128 | 4.8 Inter-assay | 1 | 20 | 253 | 15.7 | 6.2 Inter-assay | 2 | 20 | 529 | 26.6 | 5 Inter-assay | 3 | 20 | 2683 | 150 | 5.6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail