Human Intercellular Adhesion Molecule 3, ICAM3 ELISA Kit

The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting of three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes and is thus an important molecule for the first immune response. ICAM-3 comprises five immunoglobulin-like domains and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule but also as a potent signaling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that the CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. Also, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase in cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.

Concentration | O.D. | Average | Corrected ------------------------------------------ 4000 pg/ml | 1.956 | 2.036 | 1.93 2.116 2000 pg/ml | 1.392 | 1.367 | 1.261 1.343 1000 pg/ml | 0.854 | 0.869 | 0.763 0.884 500 pg/ml | 0.517 | 0.505 | 0.399 0.494 250 pg/ml | 0.303 | 0.314 | 0.208 0.326 125 pg/ml | 0.219 | 0.216 | 0.11 0.214 62.5 pg/ml | 0.162 | 0.16 | 0.054 0.158 0 pg/ml | 0.113 | 0.106 | 0 0.1

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 341 | 13.3 | 3.9 Intra-assay | 2 | 20 | 1004 | 67.2 | 6.7 Intra-assay | 3 | 20 | 2642 | 73.7 | 2.8 Inter-assay | 1 | 20 | 342 | 18.5 | 5.4 Inter-assay | 2 | 20 | 995 | 60.6 | 6.1 Inter-assay | 3 | 20 | 2660 | 116 | 4.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail