Human Lymphokine-Activated Killer T-Cell-Originated Protein Kinase, PBK ELISA Kit

PDZ binding kinase (PBK), also known as TOPK (T-LAK cell-originated protein kinase), is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family, and has all the characteristic protein kinase subdomains and a C-terminal PDZ-binding T/SXV motif. PBK is expressed in the testis restrictedly expressed in outer cell layer of seminiferous tubules, as well as placenta. PBK may be enrolled in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. This mitotic kinase phosphorylates MAP kinase p38 and seems to be active in mitosis. When phosphorylated, PBK forms a protein-protein interaction with tumor suppressor p53 (TP53), leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. The expression level of PBK is thus upregulated in a variety of neoplasms including hematological malignancies.

Concentration | O.D. | Average | Corrected ------------------------------------------ 4000 pg/ml | 1.46 | 1.492 | 1.369 1.523 2000 pg/ml | 1.014 | 1.012 | 0.889 1.009 1000 pg/ml | 0.61 | 0.646 | 0.523 0.681 500 pg/ml | 0.443 | 0.417 | 0.294 0.39 250 pg/ml | 0.288 | 0.277 | 0.154 0.266 125 pg/ml | 0.197 | 0.202 | 0.079 0.206 62.5 pg/ml | 0.151 | 0.162 | 0.039 0.172 0 pg/ml | 0.126 | 0.123 | 0 0.12

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 274 | 10.6 | 3.9 Intra-assay | 2 | 20 | 740 | 16.8 | 2.3 Intra-assay | 3 | 20 | 2644 | 78.1 | 3 Inter-assay | 1 | 20 | 272 | 22 | 8.1 Inter-assay | 2 | 20 | 735 | 59.3 | 8.1 Inter-assay | 3 | 20 | 2618 | 161 | 6.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail