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Human Prostate-Specific Antigen, KLK3 ELISA Kit

Product Specifications

Background

KLK3 (Kallikrein Related Peptidase 3) is a Protein Coding gene. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. It encodes a single-chain glycoprotein, a protease that is synthesized in the epithelial cells of the prostate gland and is present in seminal plasma. KLK3, also known as Prostate Specific Antigen (PSA), kallikrein-related peptidase 3, Gamma-seminoprotein, is a secreted protein of the glandular kallikrein subfamily of serine proteases. KLK3 contains one peptidase S1 domain. KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Product Name Alternative

Prostate-specific antigen

Synonyms

APS; KLK2A1; Psa; Semenogelase; Seminin

Gene Name

KLK3

Gene ID

354

UniProt

P07288

Target

KLK3

Field of Research

Neuroscience, Cancer, Signal transduction

Assay Type

Sandwich

Assay Protocol

Concentration | O.D. | Average | Corrected ------------------------------------------ 4000 pg/ml | 2.617 | 2.5 | 2.372 2.383 2000 pg/ml | 1.401 | 1.409 | 1.281 1.418 1000 pg/ml | 0.835 | 0.817 | 0.689 0.8 500 pg/ml | 0.427 | 0.441 | 0.313 0.456 250 pg/ml | 0.271 | 0.288 | 0.16 0.305 125 pg/ml | 0.214 | 0.203 | 0.075 0.192 62.5 pg/ml | 0.16 | 0.165 | 0.037 0.171 0 pg/ml | 0.137 | 0.128 | 0 0.12

Assay Performance Time

3.5h

Sample Type

Serum, plasma, cell culture supernates

Detection Range

62.5-4000 pg/mL

Precision

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 292 | 5 | 1.7 Intra-assay | 2 | 20 | 1125 | 21.7 | 1.9 Intra-assay | 3 | 20 | 3039 | 44.9 | 1.5 Inter-assay | 1 | 20 | 289 | 21.5 | 7.4 Inter-assay | 2 | 20 | 1118 | 54.2 | 4.9 Inter-assay | 3 | 20 | 3058 | 114 | 3.7

Sensitivity

24.7 pg/mL

Components

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail

Storage Temperature

2-8°C

Species

Human

Mass

28741

Available Sizes

Frequently Asked Questions

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