Human Prostate-Specific Antigen, KLK3 ELISA Kit
Product Specifications
Background
KLK3 (Kallikrein Related Peptidase 3) is a Protein Coding gene. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. It encodes a single-chain glycoprotein, a protease that is synthesized in the epithelial cells of the prostate gland and is present in seminal plasma. KLK3, also known as Prostate Specific Antigen (PSA), kallikrein-related peptidase 3, Gamma-seminoprotein, is a secreted protein of the glandular kallikrein subfamily of serine proteases. KLK3 contains one peptidase S1 domain. KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy
Product Name Alternative
Prostate-specific antigen
Synonyms
APS; KLK2A1; Psa; Semenogelase; Seminin
Gene Name
KLK3
Gene ID
354
UniProt
P07288
Target
KLK3
Field of Research
Neuroscience, Cancer, Signal transduction
Assay Type
Sandwich
Assay Protocol
Concentration | O.D. | Average | Corrected ------------------------------------------ 4000 pg/ml | 2.617 | 2.5 | 2.372 2.383 2000 pg/ml | 1.401 | 1.409 | 1.281 1.418 1000 pg/ml | 0.835 | 0.817 | 0.689 0.8 500 pg/ml | 0.427 | 0.441 | 0.313 0.456 250 pg/ml | 0.271 | 0.288 | 0.16 0.305 125 pg/ml | 0.214 | 0.203 | 0.075 0.192 62.5 pg/ml | 0.16 | 0.165 | 0.037 0.171 0 pg/ml | 0.137 | 0.128 | 0 0.12
Assay Performance Time
3.5h
Sample Type
Serum, plasma, cell culture supernates
Detection Range
62.5-4000 pg/mL
Precision
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 292 | 5 | 1.7 Intra-assay | 2 | 20 | 1125 | 21.7 | 1.9 Intra-assay | 3 | 20 | 3039 | 44.9 | 1.5 Inter-assay | 1 | 20 | 289 | 21.5 | 7.4 Inter-assay | 2 | 20 | 1118 | 54.2 | 4.9 Inter-assay | 3 | 20 | 3058 | 114 | 3.7
Sensitivity
24.7 pg/mL
Components
Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail
Storage Temperature
2-8°C
Species
Human
Mass
28741
Available Sizes
Frequently Asked Questions
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