Human Plasma Protease C1 Inhibitor, SERPING1 ELISA Kit

Plasma protease C1 inhibitor, also known as C1-inhibiting factor, C1-INH, C1 esterase inhibitor, SERPING1 and C1IN, is a serine proteinase inhibitor (serpin) that regulates activation of both the complement and contact systems. By its C-terminal part (serpin domain), characterized by three beta-sheets and an exposed mobile reactive loop, C1-INH binds, and blocks the activity of its target proteases. The N-terminal end (nonserpin domain) confers to C1-INH the capacity to bind lipopolysaccharides and E-selectin. Owing to this moiety, C1-INH intervenes in regulation of the inflammatory reaction. The heterozygous deficiency of C1-INH results in hereditary angioedema (HAE) . Owing to its ability to modulate the contact and complement systems and the convincing safety profile, plasma-derived C1 inhibitor is an attractive therapeutic protein to treat inflammatory diseases other than HAE. Deficiency of C1 inhibitor results in hereditary angioedema, which is characterized by recurrent episodes of localized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa. C1 inhibitor may prove useful in a variety of other diseases including septic shock, reperfusion injury, hyperacute transplant rejection, traumatic and hemorrhagic shock, and the increased vascular permeability associated with thermal injury, interleukin-2 therapy and cardiopulmonary bypass.

Concentration | O.D. | Average | Corrected ------------------------------------------ 8000 pg/ml | 2.17 | 2.16 | 2.056 2.149 4000 pg/ml | 1.145 | 1.151 | 1.047 1.156 2000 pg/ml | 0.587 | 0.626 | 0.522 0.664 1000 pg/ml | 0.333 | 0.357 | 0.253 0.38 500 pg/ml | 0.228 | 0.217 | 0.113 0.205 250 pg/ml | 0.164 | 0.158 | 0.054 0.151 125 pg/ml | 0.119 | 0.128 | 0.024 0.136 0 pg/ml | 0.101 | 0.104 | 0 0.106

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 1085 | 47.4 | 4.4 Intra-assay | 2 | 20 | 2359 | 82.4 | 3.5 Intra-assay | 3 | 20 | 5018 | 239 | 4.8 Inter-assay | 1 | 20 | 1074 | 107 | 10 Inter-assay | 2 | 20 | 2349 | 159 | 6.8 Inter-assay | 3 | 20 | 5035 | 149 | 3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail