Human Icos Ligand, ICOSLG ELISA Kit

Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. The present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production, and germinal center formation. Cancer Immunotherapy Co-stimulatory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Targets Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 4000 pg/ml | 2.702 | 2.601 | 2.494 2.5 2000 pg/ml | 1.549 | 1.48 | 1.373 1.412 1000 pg/ml | 0.77 | 0.813 | 0.706 0.856 500 pg/ml | 0.48 | 0.454 | 0.347 0.429 250 pg/ml | 0.315 | 0.296 | 0.189 0.278 125 pg/ml | 0.215 | 0.21 | 0.103 0.206 62.5 pg/ml | 0.163 | 0.166 | 0.059 0.169 0 pg/ml | 0.114 | 0.107 | 0 0.1

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 250 | 21.3 | 8.5 Intra-assay | 2 | 20 | 500 | 23.8 | 4.8 Intra-assay | 3 | 20 | 2274 | 114 | 5 Inter-assay | 1 | 20 | 252 | 22.2 | 8.8 Inter-assay | 2 | 20 | 494 | 30 | 6.1 Inter-assay | 3 | 20 | 2271 | 154 | 6.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail