Human Ribonuclease Pancreatic, RNASE1 ELISA Kit

RNase A, also known as ribonuclease A and RNASE1, belongs to ribonuclease A superfamily. It is a pancreatic-type of secretory ribonuclease. RNase A is a basic protein and its many positive charges are consistent with its binding to RNA (a poly-anion) . More generally, RNase A is unusually polar or, rather, unusually lacking in hydrophobic groups, especially aliphatic ones. As an endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly (C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Concentration | O.D. | Average | Corrected ------------------------------------------ 4000 pg/ml | 2.356 | 2.436 | 2.339 2.516 2000 pg/ml | 1.42 | 1.408 | 1.311 1.396 1000 pg/ml | 0.791 | 0.743 | 0.646 0.694 500 pg/ml | 0.409 | 0.406 | 0.309 0.403 250 pg/ml | 0.247 | 0.252 | 0.155 0.257 125 pg/ml | 0.167 | 0.171 | 0.074 0.175 62.5 pg/ml | 0.157 | 0.147 | 0.05 0.137 0 pg/ml | 0.091 | 0.097 | 0 0.103

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 428 | 42.7 | 10 Intra-assay | 2 | 20 | 1259 | 42.4 | 3.4 Intra-assay | 3 | 20 | 2928 | 97.3 | 3.3 Inter-assay | 1 | 20 | 426 | 40.4 | 9.5 Inter-assay | 2 | 20 | 1269 | 98.7 | 7.8 Inter-assay | 3 | 20 | 2905 | 229 | 7.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail