Zika Virus (Zikv) (Strain Zika Sph2015) Zikv-Ns1 ELISA Kit

Zika virus NS1 antigen is one of seven non-structural proteins. NS1 is involved in RNA replication. The possible effects of NS1 on hosts include: localizes to host cell surface and secreted extracellularly, modulates signalling of the innate immune system, has possible damages to platelets and endothelial cells through anti-NS1 antibodies.

Concentration | O.D. | Average | Corrected ------------------------------------------ 4000 pg/ml | 2.247 | 2.226 | 2.133 2.205 2000 pg/ml | 1.214 | 1.246 | 1.153 1.278 1000 pg/ml | 0.707 | 0.678 | 0.585 0.65 500 pg/ml | 0.375 | 0.364 | 0.271 0.354 250 pg/ml | 0.257 | 0.249 | 0.156 0.242 125 pg/ml | 0.169 | 0.168 | 0.075 0.167 62.5 pg/ml | 0.126 | 0.131 | 0.038 0.136 0 pg/ml | 0.1 | 0.093 | 0 0.087

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 388 | 34.8 | 9 Intra-assay | 2 | 20 | 1251 | 46.6 | 3.7 Intra-assay | 3 | 20 | 2908 | 140 | 4.8 Inter-assay | 1 | 20 | 383 | 12.5 | 3.3 Inter-assay | 2 | 20 | 1239 | 108 | 8.8 Inter-assay | 3 | 20 | 2892 | 130 | 4.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail