Mouse Thrombospondin Type-1 Domain-Containing Protein 1, Thsd1 ELISA Kit

Thrombospondin type-1 domain-containing protein 1, also known as transmembrane molecule with thrombospondin module, THSD1 and TMTSP, is a single-pass type I membrane protein that contains one TSP type-1 domain. THSD1 is a multi-domain, multi-functional glycoprotein synthesized by many cells. Matricellular THSD1 modulates cell adhesion and proliferation. It is involved in angiogenesis, inflammation, wound healing and cancer. In vitro, nanomolar concentrations of Thrombospondin-1 are required to alter endothelial and vascular smooth muscle cell adhesion, proliferation, motility, and survival. As a major platelet protein, for a long time it was postulated to control hemostasis via platelet aggregate stabilization. THSD1 is a potent angiogenesis inhibitor, and down-regulation of THSD1 has been suggested to alter tumor growth by modulating angiogenesis in a variety of tumor types.

Concentration | O.D. | Average | Corrected ------------------------------------------ 4000 pg/ml | 2.417 | 2.302 | 2.215 2.188 2000 pg/ml | 1.407 | 1.363 | 1.276 1.32 1000 pg/ml | 0.733 | 0.772 | 0.685 0.811 500 pg/ml | 0.403 | 0.421 | 0.334 0.44 250 pg/ml | 0.245 | 0.252 | 0.165 0.259 125 pg/ml | 0.174 | 0.165 | 0.078 0.157 62.5 pg/ml | 0.123 | 0.12 | 0.033 0.118 0 pg/ml | 0.091 | 0.087 | 0 0.083

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 174 | 11.9 | 6.8 Intra-assay | 2 | 20 | 623 | 28.6 | 4.6 Intra-assay | 3 | 20 | 2708 | 105 | 3.9 Inter-assay | 1 | 20 | 173 | 15.5 | 8.9 Inter-assay | 2 | 20 | 616 | 33.2 | 5.4 Inter-assay | 3 | 20 | 2739 | 191 | 7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail