Human Basal Cell Adhesion Molecule, BCAM ELISA Kit

The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha (5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3500 pg/ml | 2.608 | 2.553 | 2.42 2.497 1750 pg/ml | 1.375 | 1.426 | 1.293 1.477 875 pg/ml | 0.788 | 0.748 | 0.615 0.708 437.5 pg/ml | 0.455 | 0.428 | 0.295 0.401 218.75 pg/ml | 0.261 | 0.278 | 0.145 0.295 109.375 pg/ml | 0.21 | 0.207 | 0.074 0.203 54.6875 pg/ml | 0.187 | 0.177 | 0.044 0.167 0 pg/ml | 0.125 | 0.133 | 0 0.141

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 423 | 20.6 | 4.9 Intra-assay | 2 | 20 | 1113 | 47.4 | 4.3 Intra-assay | 3 | 20 | 2530 | 217 | 8.6 Inter-assay | 1 | 20 | 426 | 27.1 | 6.4 Inter-assay | 2 | 20 | 1123 | 84 | 7.5 Inter-assay | 3 | 20 | 2554 | 176 | 6.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail