E. Coli Aconitate Hydratase B, acnB ELISA Kit

Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3500 pg/ml | 2.268 | 2.225 | 2.102 2.183 1750 pg/ml | 1.314 | 1.297 | 1.174 1.281 875 pg/ml | 0.746 | 0.728 | 0.605 0.71 437.5 pg/ml | 0.42 | 0.418 | 0.295 0.417 218.75 pg/ml | 0.274 | 0.269 | 0.146 0.265 109.375 pg/ml | 0.208 | 0.199 | 0.076 0.191 54.6875 pg/ml | 0.168 | 0.158 | 0.035 0.149 0 pg/ml | 0.115 | 0.123 | 0 0.132

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 112 | 7.9 | 7 Intra-assay | 2 | 20 | 562 | 24.7 | 4.4 Intra-assay | 3 | 20 | 1937 | 89.5 | 4.6 Inter-assay | 1 | 20 | 112 | 8.5 | 7.6 Inter-assay | 2 | 20 | 556 | 34.9 | 6.3 Inter-assay | 3 | 20 | 1929 | 128 | 6.6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail