Human Serine Protease 27, PRSS27 ELISA Kit

The name "Pancreasin" because it is transcribed strongly in the pancreas. This secreted, tryptic serine protease, also known as Marapsin or PRSS27 (Protease, serine, 27), is a member of the peptidase S1 family. Pancreasin is inhibited by benzamidine and leupeptin but resists several classic inhibitors of trypsin. Marapsin was constitutively expressed in nonkeratinizing stratified squamous epithelia of human esophagus, tonsil, cervix, larynx, and cornea. In fact, marapsin was the second most strongly up-regulated protease in psoriatic lesions, where expression was localized to the upper region of the hyperplastic epidermis. Similarly, in the hyperproliferative epithelium of regenerating murine skin wounds, marapsin localized to the suprabasal layers, where keratinocytes undergo squamous differentiation. Marapsin's restricted expression, localization, and cytokine-inducible expression suggest a role in the terminal differentiation of keratinocytes in hyperproliferating squamous epithelia.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3200 pg/ml | 2.752 | 2.71 | 2.594 2.667 1600 pg/ml | 1.518 | 1.497 | 1.381 1.475 800 pg/ml | 0.731 | 0.783 | 0.667 0.835 400 pg/ml | 0.46 | 0.449 | 0.333 0.438 200 pg/ml | 0.293 | 0.285 | 0.169 0.276 100 pg/ml | 0.202 | 0.196 | 0.08 0.189 50 pg/ml | 0.149 | 0.153 | 0.037 0.156 0 pg/ml | 0.116 | 0.116 | 0 0.115

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 231 | 8.8 | 3.8 Intra-assay | 2 | 20 | 796 | 27.2 | 3.4 Intra-assay | 3 | 20 | 2153 | 77.5 | 3.6 Inter-assay | 1 | 20 | 233 | 13.6 | 5.9 Inter-assay | 2 | 20 | 793 | 36.1 | 4.5 Inter-assay | 3 | 20 | 2165 | 82.9 | 3.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail