Human Epidermal Growth Factor Receptor, EGFR ELISA Kit

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT) . In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.608 | 2.683 | 2.593 2.757 1500 pg/ml | 1.529 | 1.48 | 1.39 1.43 750 pg/ml | 0.78 | 0.772 | 0.682 0.763 375 pg/ml | 0.414 | 0.438 | 0.348 0.461 187.5 pg/ml | 0.264 | 0.261 | 0.171 0.257 93.75 pg/ml | 0.181 | 0.171 | 0.081 0.16 46.875 pg/ml | 0.135 | 0.13 | 0.04 0.124 0 pg/ml | 0.091 | 0.09 | 0 0.088

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 138 | 12.4 | 9 Intra-assay | 2 | 20 | 574 | 21.8 | 3.8 Intra-assay | 3 | 20 | 1793 | 83.5 | 4.7 Inter-assay | 1 | 20 | 139 | 4.3 | 3.1 Inter-assay | 2 | 20 | 572 | 50.9 | 8.9 Inter-assay | 3 | 20 | 1776 | 82.6 | 4.6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail