Human Neural Cell Adhesion Molecule L1, L1CAM ELISA Kit

L1 cell adhesion molecule (L1CAM), also designated as CD171, is a cell adhesion receptor of the immunoglobulin superfamily, known for its roles in nerve cell function. While originally believed to be present only in brain cells, in recent years L1-CAM has been detected in other tissues, and a variety of cancer cells, including some common types of human cancer. L1CAM interacts with a variety of ligands including axonin-1, CD9, neurocan, and integrins, and it has been revealed that the RGD motif in the sixth Ig domain of L1CAM is a binding site for integrins, thus important for nuclear signaling. Disruption of L1CAM function causes three X-linked neurological syndromes, i.e. hydrocephalus, MASA syndrome (mental retardation, aphasia, shuffling gait, and adducted thumbs), and spastic paraplegia syndrome. Overexpression of L1CAM in normal and cancer cells increased motility, enhanced growth rate, and promoted cell transformation and tumorigenicity. Recent work has identified L1CAM (CD171) as a novel marker for human carcinoma progression, and a candidate for anti-cancer therapy.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.58 | 2.584 | 2.465 2.589 1500 pg/ml | 1.438 | 1.49 | 1.371 1.543 750 pg/ml | 0.77 | 0.804 | 0.685 0.839 375 pg/ml | 0.437 | 0.449 | 0.33 0.462 187.5 pg/ml | 0.253 | 0.268 | 0.149 0.284 93.75 pg/ml | 0.176 | 0.183 | 0.064 0.19 46.875 pg/ml | 0.131 | 0.136 | 0.017 0.142 0 pg/ml | 0.119 | 0.119 | 0 0.12

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 340 | 31.5 | 9.3 Intra-assay | 2 | 20 | 895 | 89.7 | 10 Intra-assay | 3 | 20 | 1865 | 180 | 9.7 Inter-assay | 1 | 20 | 341 | 38.4 | 11.2 Inter-assay | 2 | 20 | 899 | 95.4 | 10.6 Inter-assay | 3 | 20 | 1883 | 169 | 9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail