Human Interleukin-37, IL37 ELISA Kit

Interleukin 1 family member 7, or interleukin 37 (IL1F7 / IL37 / IL-1H4) is a secretory protein belonging to the Interleukin 1 family. IL-1F7 was localized in human peripheral monocytic cells. It has been localized the expression of IL-1F7b protein in discrete cell populations including plasma cells and tumor cells. These data suggest that IL-1F7 may be involved in immune response, inflammatory diseases, and/or cancer. Through constructing an adenoviral vector that allows high-level expression in murine and human cells, it has been demonstrated that the ability of adenovirus-mediated gene transfer of IL1F7 to induce an IL-12- and Fas ligand-dependent anti-tumor response. Complete inhibition of tumor growth was observed following multiple injections of IL1F7 in most animals. These results suggest that IL1F7 could play a role in both innate and adaptive immune responses, similar to IL-18. Moreover, IL1F7 could be useful for cancer gene therapy.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.139 | 2.252 | 2.146 2.365 1500 pg/ml | 1.287 | 1.302 | 1.196 1.317 750 pg/ml | 0.687 | 0.718 | 0.612 0.749 375 pg/ml | 0.459 | 0.43 | 0.324 0.401 187.5 pg/ml | 0.288 | 0.271 | 0.165 0.254 93.75 pg/ml | 0.195 | 0.19 | 0.084 0.185 46.875 pg/ml | 0.138 | 0.146 | 0.04 0.154 0 pg/ml | 0.099 | 0.106 | 0 0.113

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 225 | 10.6 | 4.7 Intra-assay | 2 | 20 | 1023 | 29.6 | 2.9 Intra-assay | 3 | 20 | 2274 | 55.1 | 2.4 Inter-assay | 1 | 20 | 226 | 14.9 | 6.6 Inter-assay | 2 | 20 | 1012 | 47.2 | 4.7 Inter-assay | 3 | 20 | 2281 | 112 | 4.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail