Human Plasminogen Activator Inhibitor 1, SERPINE1 ELISA Kit

Plasminogen activator inhibitor 1, also known as PAI-1, Endothelial plasminogen activator inhibitor, SerpinE1 and PLANH1, is a secreted glycoprotein that belongs to the serpin family. SerpinE1 is the primary physiological inhibitor of the two plasminogen activators urokinase (uPA) and tissue plasminogen activator (tPA) . Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Defects in SerpinE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency) which is characterized by abnormal bleeding due to SerpinE1 defect in the plasma. High concentrations of SerpinE1 have been associated with thrombophilia which is an autosomal dominant disorder in which affected individuals are prone to develop serious spontaneous thrombosis. Studies of PAI-1 have contributed significantly to the elucidation of the protease inhibitory mechanism of serpins, which is based on a metastable native state becoming stabilised by insertion of the RCL into the central beta-sheet A and formation of covalent complexes with target proteases. Greater expression of PAI-1 has been associated with increased survival of cells and resistance to apoptosis. PAI-1 appears to influence apoptosis by decreasing cell adhesion (anoikis) as well as its effect on intracellular signaling. PAI-1, in its active state, also binds to the extracellular protein vitronectin. When in complex with its target proteases, it binds with high affinity to endocytosis receptors of the low density receptor family. The mechanisms of PAI-1 overexpression during obesity are complex, and it is conceivable that several inducers are involved at the same time at several sites of synthesis. PAI-1 is also implicated in adipose tissue development. It suggests that PAI-1 inhibitors serve in the control of atherothrombosis.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.027 | 2.045 | 1.926 2.062 1500 pg/ml | 1.197 | 1.233 | 1.114 1.269 750 pg/ml | 0.7 | 0.708 | 0.589 0.716 375 pg/ml | 0.438 | 0.44 | 0.321 0.442 187.5 pg/ml | 0.307 | 0.287 | 0.168 0.267 93.75 pg/ml | 0.216 | 0.202 | 0.083 0.188 46.875 pg/ml | 0.158 | 0.16 | 0.041 0.161 0 pg/ml | 0.124 | 0.119 | 0 0.113

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 146 | 5.2 | 3.5 Intra-assay | 2 | 20 | 584 | 16.1 | 2.8 Intra-assay | 3 | 20 | 2246 | 71.1 | 3.2 Inter-assay | 1 | 20 | 146 | 13.3 | 9.1 Inter-assay | 2 | 20 | 586 | 49.1 | 8.4 Inter-assay | 3 | 20 | 2260 | 199 | 8.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail