Human Alpha-1-Antichymotrypsin, SERPINA3 ELISA Kit

SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors consisting of at least 35 members. SerpinA3 has been demonstrated to inhibit the activity of certain serine proteases, such as cathepsin G found in neutrophils, and chymases present in mast cells, by inducing a major conformational rearrangement, and thus protects some tissues from damage caused by proteolytic enzymes. This enzyme is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. Besides, ACT gene polymorphism has been implicated with Alzheimer’s disease (AD), cerebral amyloid angiopathy (CAA), as well as stroke, since SerpinA3 is a major constituent of the plaques in AD and an inhibitor of amyloid beta peptide degradation.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.436 | 2.322 | 2.191 2.207 1500 pg/ml | 1.289 | 1.225 | 1.094 1.16 750 pg/ml | 0.659 | 0.661 | 0.53 0.662 375 pg/ml | 0.395 | 0.388 | 0.257 0.38 187.5 pg/ml | 0.242 | 0.255 | 0.124 0.267 93.75 pg/ml | 0.205 | 0.197 | 0.066 0.188 46.875 pg/ml | 0.164 | 0.163 | 0.032 0.161 0 pg/ml | 0.133 | 0.131 | 0 0.128

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 135 | 6.1 | 4.5 Intra-assay | 2 | 20 | 409 | 21.4 | 5.2 Intra-assay | 3 | 20 | 1513 | 95.9 | 6.3 Inter-assay | 1 | 20 | 134 | 8.4 | 6.3 Inter-assay | 2 | 20 | 414 | 21.3 | 5.1 Inter-assay | 3 | 20 | 1504 | 73 | 4.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail