Human Complement Factor H, CFH ELISA Kit

Complement factor H, also known as H factor 1, and CFH, is a sialic acid containing glycoprotein that plays an integral role in the regulation of the complement-mediated immune system that is involved in microbial defense, immune complex processing, and programmed cell death. Factor H protects host cells from injury resulting from unrestrained complement activation. CFH regulates complement activation on self cells by possessing both cofactor activity for the Factor I mediated C3b cleavage, and decay accelerating activity against the alternative pathway C3 convertase, C3bBb. CFH protects self cells from complement activation but not bacteria/viruses. Due to the central role that CFH plays in the regulation of complement, there are many clinical implications arrising from aberrant CFH activity. Mutations in the Factor H gene are associated with severe and diverse diseases including the rare renal disorders hemolytic uremic syndrome (HUS) and membranoproliferative glomerulonephritis (MPGN) also termed dense deposit disease (DDD), membranoproliferative glomuleronephritis type II or dense deposit disease, as well as the more frequent retinal disease age related macular degeneration (AMD) . In addition to its complement regulatory activities, factor H has multiple physiological activities and 1) acts as an extracellular matrix component, 2) binds to cellular receptors of the integrin type, and 3) interacts with a wide selection of ligands, such as the C-reactive protein, thrombospondin, bone sialoprotein, osteopontin, and heparin.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2500 pg/ml | 2.903 | 2.782 | 2.686 2.661 1250 pg/ml | 1.329 | 1.336 | 1.24 1.344 625 pg/ml | 0.615 | 0.63 | 0.534 0.646 312.5 pg/ml | 0.356 | 0.342 | 0.246 0.329 156.25 pg/ml | 0.2 | 0.215 | 0.119 0.23 78.125 pg/ml | 0.146 | 0.148 | 0.052 0.151 39.0625 pg/ml | 0.128 | 0.124 | 0.028 0.121 0 pg/ml | 0.097 | 0.096 | 0 0.096

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 386 | 19.9 | 5.2 Intra-assay | 2 | 20 | 772 | 28.7 | 3.7 Intra-assay | 3 | 20 | 1883 | 85.2 | 4.5 Inter-assay | 1 | 20 | 383 | 38 | 9.9 Inter-assay | 2 | 20 | 766 | 76.1 | 9.9 Inter-assay | 3 | 20 | 1871 | 157 | 8.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail