Human Protein Disulfide-Isomerase, P4HB ELISA Kit

Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein that belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp12 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity) . P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.141 | 2.124 | 1.997 2.106 1500 pg/ml | 1.163 | 1.103 | 0.976 1.042 750 pg/ml | 0.647 | 0.644 | 0.517 0.64 375 pg/ml | 0.391 | 0.39 | 0.263 0.389 187.5 pg/ml | 0.272 | 0.265 | 0.138 0.257 93.75 pg/ml | 0.21 | 0.198 | 0.071 0.186 46.875 pg/ml | 0.17 | 0.161 | 0.034 0.152 0 pg/ml | 0.123 | 0.127 | 0 0.131

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 227 | 10.8 | 4.8 Intra-assay | 2 | 20 | 907 | 39.7 | 4.4 Intra-assay | 3 | 20 | 2388 | 68.6 | 2.9 Inter-assay | 1 | 20 | 228 | 23.5 | 10.3 Inter-assay | 2 | 20 | 913 | 70.1 | 7.7 Inter-assay | 3 | 20 | 2414 | 161 | 6.7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail