Human Acrosomal Protein Sp-10, ACRV1 ELISA Kit

Acrosomal protein SP-1, also known as Acrosomal vesicle protein 1 and ACRV1, is a testis-specific, differentiation antigen, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. Regulation of cell type-specific gene transcription is central to cellular differentiation and development. During spermatogenesis, a number of testis-specific genes are expressed in a precise spatiotemporal order. The longest transcript of ACRV1 / SP-1 is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. ACRV1 / SP-1 is a testis-specific acrosomal protein that has been detected in several species including humans. It may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans. ACRV1 / SP-1 may be involved in sperm-zona binding or penetration. It is also a intra-acrosomal protein that is considered to be a vaccine candidate for immunocontraception.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.678 | 2.702 | 2.575 2.726 1500 pg/ml | 1.32 | 1.283 | 1.156 1.246 750 pg/ml | 0.708 | 0.713 | 0.586 0.718 375 pg/ml | 0.41 | 0.414 | 0.287 0.417 187.5 pg/ml | 0.272 | 0.256 | 0.129 0.24 93.75 pg/ml | 0.187 | 0.193 | 0.066 0.199 46.875 pg/ml | 0.145 | 0.155 | 0.028 0.165 0 pg/ml | 0.129 | 0.127 | 0 0.125

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 187 | 17.4 | 9.3 Intra-assay | 2 | 20 | 533 | 52.8 | 9.9 Intra-assay | 3 | 20 | 1524 | 149 | 9.8 Inter-assay | 1 | 20 | 185 | 20.7 | 11.2 Inter-assay | 2 | 20 | 530 | 56.7 | 10.7 Inter-assay | 3 | 20 | 1508 | 136 | 9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail