Human Ribonucleoside-Diphosphate Reductase Subunit M2 B, RRM2B ELISA Kit

Ribonucleoside reductase subunit M2B, also known as RRM2B or p53R2, is an enzyme belonging to the iron-dependent ribonucleotide reductase (RNR) enzyme family which is essential for DNA synthesis. Ribonucleotide reductase (RNR) is an enzyme that catalyzes the formation of deoxyribonucleotides from ribonucleotides and plays a critical role in regulating the total rate of DNA synthesis so that DNA to cell mass is maintained at a constant ratio during cell division and DNA repair. RRM2B is a phosphorylated protein. It is hypothesized that RRM2B activity can be regulated at the posttranslational level in response to DNA damage. RRM2B has previously been shown to be essential for the maintenance of mtDNA copy number and its candidacy for tumor suppression has been evaluated in several mutational analyses of different cancer types. However, the contribution of RRM2B to the DNA damage response has been questioned because its transcriptional induction upon DNA damage is not rapid enough for prompt DNA repair. Instead, ATM-mediated phosphorylation has been suggested to regulate the DNA repair activity of RRM2B posttranslationally. Besides, a defect in RRM2B can induce a mild muscle disease of adult onset through disturbance of mitochondrial homeostasis but that this defect does not appear to be oncogenic.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.358 | 2.34 | 2.257 2.322 1500 pg/ml | 1.169 | 1.201 | 1.118 1.233 750 pg/ml | 0.579 | 0.615 | 0.532 0.651 375 pg/ml | 0.324 | 0.334 | 0.251 0.344 187.5 pg/ml | 0.199 | 0.203 | 0.12 0.207 93.75 pg/ml | 0.129 | 0.135 | 0.052 0.141 46.875 pg/ml | 0.121 | 0.116 | 0.033 0.111 0 pg/ml | 0.085 | 0.083 | 0 0.081

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 201 | 6 | 3 Intra-assay | 2 | 20 | 743 | 27.3 | 3.7 Intra-assay | 3 | 20 | 1517 | 51.6 | 3.4 Inter-assay | 1 | 20 | 202 | 13.1 | 6.5 Inter-assay | 2 | 20 | 741 | 44.1 | 6 Inter-assay | 3 | 20 | 1511 | 81.6 | 5.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail