Mouse Interleukin-18, Il18 ELISA Kit

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.646 | 2.591 | 2.466 2.537 1500 pg/ml | 1.409 | 1.368 | 1.243 1.327 750 pg/ml | 0.716 | 0.745 | 0.62 0.775 375 pg/ml | 0.411 | 0.433 | 0.308 0.456 187.5 pg/ml | 0.265 | 0.275 | 0.15 0.285 93.75 pg/ml | 0.204 | 0.2 | 0.075 0.196 46.875 pg/ml | 0.148 | 0.158 | 0.033 0.169 0 pg/ml | 0.124 | 0.125 | 0 0.126

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 308 | 23.6 | 7.7 Intra-assay | 2 | 20 | 810 | 47.7 | 5.9 Intra-assay | 3 | 20 | 1977 | 80.6 | 4.1 Inter-assay | 1 | 20 | 311 | 16 | 5.1 Inter-assay | 2 | 20 | 807 | 42.8 | 5.3 Inter-assay | 3 | 20 | 1966 | 100 | 5.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail