Mouse Cytokine Receptor Common Subunit Gamma, Il2rg ELISA Kit

The common gamma chain (γc) (or CD132), also known as interleukin-2 receptor subunit gamma or IL2RG, is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. The common gamma chain (γc) (or IL2RG), is a cytokine receptor subunit that is common to the receptor complexes for at least six different interleukin receptors: IL-2, IL-4, IL-7, IL-9, IL-15, and the interleukin-21 receptor. It is a component of multiple cytokine receptors that are essential for lymphocyte development and function. X-linked severe combined immunodeficiency (X-SCID) is a rare and potentially fatal disease caused by mutations of IL2RG, the gene encoding IL2RG. IL2RG was demonstrated to be a component of the IL-4 receptor based on chemical cross-linking data, the ability of IL2RG to augment IL-4 binding affinity. The observation that IL-2R gamma is a functional component of the IL-4 receptor, together with the finding that IL-2R gamma associates with the IL-7 receptor, begins to elucidate why a deficiency of this common gamma chain (gamma c) has a profound effect on lymphoid function and development, as seen in X-linked severe combined immunodeficiency.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.082 | 2.18 | 2.058 2.279 1500 pg/ml | 1.169 | 1.113 | 0.991 1.058 750 pg/ml | 0.592 | 0.6 | 0.478 0.609 375 pg/ml | 0.368 | 0.354 | 0.232 0.34 187.5 pg/ml | 0.251 | 0.239 | 0.117 0.228 93.75 pg/ml | 0.163 | 0.173 | 0.051 0.183 46.875 pg/ml | 0.134 | 0.139 | 0.017 0.144 0 pg/ml | 0.125 | 0.122 | 0 0.12

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 200 | 5.7 | 2.9 Intra-assay | 2 | 20 | 625 | 23 | 3.7 Intra-assay | 3 | 20 | 1894 | 95.4 | 5 Inter-assay | 1 | 20 | 200 | 14.4 | 7.2 Inter-assay | 2 | 20 | 618 | 37.8 | 6.1 Inter-assay | 3 | 20 | 1916 | 139 | 7.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail