Mouse Semaphorin-4D, Sema4d ELISA Kit

Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D) . Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.333 | 2.287 | 2.198 2.242 1500 pg/ml | 1.259 | 1.209 | 1.12 1.159 750 pg/ml | 0.649 | 0.663 | 0.574 0.677 375 pg/ml | 0.389 | 0.372 | 0.283 0.355 187.5 pg/ml | 0.25 | 0.235 | 0.146 0.22 93.75 pg/ml | 0.167 | 0.162 | 0.073 0.157 46.875 pg/ml | 0.129 | 0.124 | 0.035 0.119 0 pg/ml | 0.085 | 0.089 | 0 0.093

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 141 | 6.3 | 4.5 Intra-assay | 2 | 20 | 542 | 10.4 | 1.9 Intra-assay | 3 | 20 | 1869 | 44.4 | 2.4 Inter-assay | 1 | 20 | 140 | 9.5 | 6.8 Inter-assay | 2 | 20 | 539 | 19.4 | 3.6 Inter-assay | 3 | 20 | 1850 | 75.4 | 4.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail