Human Interleukin-18-Binding Protein, IL18BP ELISA Kit

Interleukin-18-binding protein (IL-18BP) is a constitutively expressed and secreted protein. IL-18BP is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18) and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. The adjacently located family members IL18 Receptor 1 (IL18R1) and IL18 receptor accessory protein (IL18RAP) may also be important in the development of asthma and atopy. IL-18 binding protein (IL-18BP) was only moderately elevated, resulting in a high level of biologically active free IL-18 in HPS. A severe IL-18/IL-18BP imbalance results in Th-1 lymphocyte and macrophage activation, which escapes control by NK-cell cytotoxicity and may allow for secondary HPS in patients with underlying diseases.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.66 | 2.591 | 2.486 2.522 1500 pg/ml | 1.259 | 1.215 | 1.11 1.171 750 pg/ml | 0.564 | 0.598 | 0.493 0.632 375 pg/ml | 0.289 | 0.311 | 0.206 0.333 187.5 pg/ml | 0.199 | 0.193 | 0.088 0.187 93.75 pg/ml | 0.13 | 0.139 | 0.034 0.148 46.875 pg/ml | 0.112 | 0.115 | 0.01 0.118 0 pg/ml | 0.098 | 0.105 | 0 0.112

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 144 | 7.3 | 5 Intra-assay | 2 | 20 | 437 | 17.5 | 4 Intra-assay | 3 | 20 | 1900 | 104 | 5.5 Inter-assay | 1 | 20 | 145 | 13.2 | 9.1 Inter-assay | 2 | 20 | 435 | 17.2 | 4 Inter-assay | 3 | 20 | 1898 | 119 | 6.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail